Investigation of the dynamics of action optima for some physicochemical factors in the course of complex optimization of conditions for the biosynthesis of amylolytic enzymes of microbial origin
DOI:
https://doi.org/10.18372/2306-6407.1.18097Keywords:
Streptomyces recefensis var. lyticus 2P-15, nutrient medium, amylolytic enzymes, amylolytic activity, biosynthetic capacity of the strain, optimization of biosynthesis conditionsAbstract
The most important factors in increasing efficiency are the composition of the nutrient medium, which can be optimized depending on the needs of microorganisms, and physicochemical factors that directly affect both the biosynthesis of amylolytic enzymes by the studied strain and the biosynthetic capacity of the strain in general. The aim of this work is to optimize the biosynthetic capacity of Streptomyces recifensis var. lyticus 2P-15 strain in terms of amylolytic enzyme synthesis. The object of study is the strain of Streptomyces recifensis var. lyticus 2P-15, obtained by three-stage selection of the producer. The simplex method of selecting the composition of the medium was used to perform the study.
References
Pyrog T. P., Penchuk Yu. M. Biochemical basics of microbial synthesis: textbook. – K.: Lira-K Publishing House, 2020. – 258 p.
Paul J. S., Lall B. M., Jadhav S. K. Parameter’s optimization and kinetics study of $ $ amylase enzyme of Bacillus sp. MB6 isolated from vegetable // Biochemistry. – 2017. – Vol. 52. – P. 123–129. https://doi.org/10.1016/j.procbio.2016.10.005
Saha S. P., Ghosh S., Mazumdar D., Sarbobhouma Ghosh, Dipanwita Ghosh, Mahima Misti Sarkar, Swarnendu Roy Valorization of banana peel into $ $ amylase using one factor at a time (OFAT) assisted artificial neural network (ANN) and its partial purification, characterization, and kinetics study // Food Bioscience. – 2023. – Vol. 53, 102533. https://doi.org/10.1016/j.fbio.2023.102533