ENZIME ACTIVITY OF HIGHER BASIDIOMYCETES MUSHROOM SCHIZOPHYLLUM COMMUNE
DOI:
https://doi.org/10.18372/2306-1472.52.2369Keywords:
complex liquid nourishing mediums, endo-1, 4-B-glucanase, exoglucanase, hydrolytic enzymes, monophenolmonooxygenase, qualitative color enzyme reactions, reducing substances, Schizophyllum communeAbstract
The purpose of this work was a revelation and evaluation of spectrum and activity of hydrolytic enzymes of higher basidiomycetes Schizophyllum commune in a surface and submerged culture. 21 strains of S. commune were object of investigation. Researches were conducted by standard microbiological, biochemical and biotechnological methods. All strains on agar mediums were shown the following enzymes: amylase, caseinase, gelatinase, polygalacturonase, pectattranselyminase, urease, lipase, cellulase, laccase and peroxydase. The demonstration of oxidizing enzymes of laccase and peroxydase depended on composition of medium. The estimation of presence and level of activity of endo-1,4-b-glucanase, exoglucanase and monophenolmonooxygenase at submerged cultivation indicate primary influence of components of complex nourishing medium on enzyme activity of strain 1760 S. commune.
References
Hummel K.M. Extracellular protease production by submerged cultures of Schizophyllum commune / Katrina M. Hummel, Amy L. Inselman, Erica R. Ramos et all // Mycologia. 1998. Vol. 90, N 5. - P. 883–889.
Pandee P. Production and properties of a fibrinolytic enzyme by Schizophyllum commune BL23 / Patcharaporn Pandee, Aran H-Kittikul, Ohsugi et all // Songklanakarin J. Sci. Technol. - 2008. – Vol. 30, N 4. – P. 447 453.
Subramaniyan S. Biotechnology of Microbial Xylanases: Enzymology, Molecular Biology, Application / S. Subramaniyan, P. Prema // Critical Reviews in Biotechnology. 2002. – Vol. 22, N 1. – Р. 33–46.
Kolenová K. Purification and characteriza-tion of two minor endo-β-1,4-xylanases of Schizophyllum commune / Katarína Kolenová, Mária Vršanská, Peter Biely // Enzyme and Microbial Technology. - 2005. - Vol. 36, N 7. – P. 903 910.
Wu J. -glucosidase derived from Schizophyllum commune an efficient enzyme of biotransformation of genistein / J. Wu, W. Yu, X. Zhang // Book of abstracts the 5th International Medicinal Mushroom Conference. Nantong, China, 5-8 September, 2009. – P. 139-140.
Baldrian P. Fungal laccases: occurrence and properties / P. Baldrian // FEMS Microbial Rev. - 2006. – Vol. 30. – P. 215-242.
Kenkebashvilli N. Effect of nutrient medium composition on laccase and manganese peroxidase activity in medicinal mushrooms / N. Kenkebashvilli, V. Elisashvilli, Y. Hadar // International Journal of Medicinal Mushrooms. - 2009. - Vol. 11, N 2. - P. 191-198.
Molitoris H.P. Physiology of marine fungi. A screening program for marine fungi / H.P. Molitoris, K. Schaumann // The biolgy of marine fungi. Eds. S.T. Moss. – Cambrige : Cambrige University Press, 1986. – P. 35-47.
Бухало А.С. Высшие съедобные базидио-мицеты в чистой культуре / А.С. Бухало. – К.: Наукова думка, 1988. – 144 с.
Дудка И.А. Методы экспериментальной микологии: справ. / И.А. Дудка, С.П. Вассер, И.А. Элланская и др. – К.: Наукова думка, 1982. – 561 с.
Тодоров И.И. Клинические лабораторные исследования в педиатрии / И.И. Тодоров. – София: Медицина и физкультура, 1968. – 230 с.
Гавриленко В.Ф. Большой практикум по физиологии растений / В.Ф. Гавриленко, М.Е. Ладыгина, Л.М. Хандобина. – М.: Высш. шк., 1975. – С. 284-285.
