Assessment of biosafety aspects during procedures of genetic transformation of plants


  • G.P. Petiukh National Aviation University
  • N.V. Karachinska Національний авіаційний університет



biosafety, genetic transformation of plants, tolerance to antibiotics, horizontal gene transfer


The article discusses the possibility of risks of uncontrolled gene transfer during procedures of plant genetic transformation, which is an integral part of the system of biosafety of the use of genetically modified organisms in their release into the environment. In order to test this phenomenon, the authors conducted research to study the likelihood of such transfer of genes from genetic constructs found in cells of bacterial strains used to transform plants, in particular Agrobacterium tumefaciens, in the form of plasmid DNA and determine tolerance to antibiotics and other bacteria cells, in particular Pseudomonas fluorescens. Bacterial cells with cross-tolerance to antibiotics were obtained in the experiments, which suggest the presence of the phenomenon of gene transfer by a horizontal mechanism. The obtained result suggests the possibility of unpredictable consequences in the agroecosystems as a result of gene drift from genetic constructs to soil microbiota.

Author Biographies

G.P. Petiukh, National Aviation University

Associate Professor, Cand. of Biol. Sc.

N.V. Karachinska, Національний авіаційний університет

Associate Professor, Cand. of Biol. Sc.


Agrobacterium-mediated transformation of five wild Solanum species using in vitro microtubers / A. Kumar, M. Miller, P.Whitty [] // Plant-Cell-Reports. – 1995. – 14, №5. – Р. 324–328.

Identification of Agrobacterium spp. present within Brassica napus seed by Taqman PCR - implications for GM screening procedures / S.A. Weller, S.A. Simpkins, D.E. Stead [] //Arch. Microbiol. –2002. – V.178. – P.338–343.

Leifert C. Microbial hazards in plant tissue and cell cultures / C.Leifert, A.C. Cassells // In Vitro Cellular &Developmental Biology-Plant. – 2001. –V.37. –P.133–138.

Leifert C. Quality assurance systems for plant cell and tissue culture: the problem of latent persistence of bacterial pathogens and Agrobacterium-based transformation vector systems / C. Leifert //Acta Hort. – 2000. – V.530. – P.87–91.

Van der Hoeven C. Latent agrobacteria detected in transgenic plants / C. van der Hoeven, A. Dietz, J. Landsmann // Nachrichtenbl.Deut. Pflanzenschutzd. – 1991. – №43. – P.249–251.

A risk assessment study of plant genetic transformation using Agrobacterium and implications for analysis of transgenic plants / C. Barrett, E. Cobb, R. Mc Nicol [et al.] // Plant Cell Tiss. Org. Cult. – 1997. –V.47. – P.135–144.

Lorenz M.G. Natural genetic transformation of Pseudomonas stutzeri by sandad sorbed DNA / M.G. Lorenz, W. Wackernagel //Arch. Microbiol.–1990.– V.154.– P.380–385.

Smit E. Plasmid transfer from Pseudomonas fluorescens to indigenous bacteria in soil using bacteriophage fR2f for donor counter selection / E. Smit, J.D. van Elsas, J.A. van Veen, W.M. de Vos //Appl. Environ. Microbiol. – 1991. – V.57. – P.3482–3488.

Construction of an intron-containing marker gene: Splicing of the intron in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation / G. Vancanneyt, R. Schmidt, A. O'Connor-Sanchez [] // Моl. Gen. Genet. – 1990. – V.220. – P. 245–250.

Егоров Н.С. Выделение микробов-антогонистов и биологические методы учета их антибиотической активности / Н.С. Егоров – М.: МГУ, 1957. – 78 с.

Quantitative selective PCR of 16S ribosomal DNA correlates well with selective agar plating in describing population dynamics of indigenous Pseudomonas spp. in soil hot spots / K. Jonsen, Q. Enger, C. Jacobsen [] // App. Envir. Microbiol. – 1999. – V.65, №4. – Р.1786–1789.





Experimental articles