Biotechnological application of methyl-β-cyclodextrin for cholesterol content manipulation in biological membranes

Authors

  • K. O. Paliienko National Aviation University
  • O. A. Vasylchenko National Aviation University
  • A. O. Pastukhov Palladin Institute of Biochemistry
  • S. V. Riabov The Institute of Macromolecular Chemistry
  • T. A. Borisova Palladin Institute of Biochemistry

DOI:

https://doi.org/10.18372/2306-6407.1.11647

Keywords:

methyl-β-cyclodextrin, cholesterol, maghemite nanoparticles, biological membranes

Abstract

Investigation is devoted to demonstrate application of methyl-β-cyclodextrin (MβCD) as cargo that attached to magnetic nanoparticles, in particularly maghemite, for the manipulation cholesterol content in synaptic membranes.

Author Biographies

K. O. Paliienko, National Aviation University

student of biotechnology department

O. A. Vasylchenko, National Aviation University

associate professor, candidate of medical science,

A. O. Pastukhov, Palladin Institute of Biochemistry

senior engineer

S. V. Riabov, The Institute of Macromolecular Chemistry

head of polymers modification department, doctor of chemical science, senior staff scientist

T. A. Borisova, Palladin Institute of Biochemistry

head of neurochemistry department, doctor of biological science, professor

References

Martens J. R. Targeting of ion channels to membrane microdomains: localization of KV channels to lipid rafts / J. R. Martens, K. O'Connell, M. Tamkun // Trends in pharmacological sciences. – 2004. – Vol. 25, № 1. – P. 16–21.

Chattopadhyay A. Lipid–protein interactions, regulation and dysfunction of brain cholesterol / Amitabha Chattopadhyay, Yamuna Paila // Biochemical and biophysical research communications. – 2007. – Vol. 354, № 3. – P. 627–633.

Methyl-beta-cyclodextrin stimulates glucose uptake in Clone 9 cells: a possible role for lipid rafts / K. Barnes, J. C. Ingram, M. D. Bennett [et al.] // Biochemical Journal. – 2004. – Vol. 378, № 2. – P. 343–351.

Effect of O-methyl-β-cyclodextrin-modified magnetic nanoparticles on the uptake and extracellular level of L-glutamate in brain nerve terminals / D. Horák, M. Beneš, Z. Procházková [et al.] // Colloids and Surfaces B: Biointerfaces. – 2017. – Vol. 149. – P. 64–71.

Взаємодія наночастинок магнетиту, покритих полімерами, з нервовими терміналями головного мозку та тромбоцитами крові / Т. О. Борисова, Н. О. Дудченко, О. Б. Брик [та ін.] // Biotechnologia Acta. – 2011. – Т. 4, № 5. – С. 45–56.

Cotman C. W. Isolation of synaptosomal and synaptic plasma membrane fractions / C. W. Cotman // Methods in enzymology. – 1974. – Vol. 31. – P. 445–452.

Larson E. Artificial reductant enhancement of the Lowry method for protein determination / E. Larson, B. Howlett, A. Jagendorf // Analytical Biochemistry. – 1986. – Vol. 155, № 2. – P. 243–248.

Zlatkis A. A new method for the direct determination of serum cholesterol / A. Zlatkis, B. Zak, A. J. Boyle // Journal of Laboratory and Clinical Medicine. – 1953. – Vol. 41. – P. 486–492.

Paliienko K. O. Maghemite (γ-Fe2O3) nanoparticles as a potential sorbent of extracellular glutamate in synaptic cleft / Kostiantyn Paliienko, Tatiana Borisova // 25th Annual conference “Modern aspects of biochemistry and biotechnology”: 2nd Conference for young scientists of the division of Biochemistry, Physiology and Molecular Biology National Academy of Sciences of Ukraine, 6-9 June 2017: Theses of reports. – Kyiv, 2017. – P. 74.

Published

2017-06-23

Issue

Section

Experimental articles