ENZIME ACTIVITY OF HIGHER BASIDIOMYCETES MUSHROOM SCHIZOPHYLLUM COMMUNE

Authors

  • А.С. Бухало M.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine
  • О.М. Дуган National Technical University of Ukraine “Kiev Polytechnic Institute”
  • М.Р. Максимюк National Aviation University
  • В.М. Ліновицька National Technical University of Ukraine “Kiev Polytechnic Institute”

DOI:

https://doi.org/10.18372/2306-1472.52.2369

Keywords:

complex liquid nourishing mediums, endo-1, 4-B-glucanase, exoglucanase, hydrolytic enzymes, monophenolmonooxygenase, qualitative color enzyme reactions, reducing substances, Schizophyllum commune

Abstract

 The purpose of this work was a revelation and evaluation of spectrum and activity of hydrolytic enzymes of higher basidiomycetes Schizophyllum commune in a surface and submerged culture. 21 strains of S. commune were object of investigation. Researches were conducted by standard microbiological, biochemical and biotechnological methods. All strains on agar mediums were shown the following enzymes: amylase, caseinase, gelatinase, polygalacturonase, pectattranselyminase, urease, lipase, cellulase, laccase and peroxydase. The demonstration of oxidizing enzymes of laccase and peroxydase depended on composition of medium. The estimation of presence and level of activity of endo-1,4-b-glucanase, exoglucanase and monophenolmonooxygenase at submerged cultivation indicate primary influence of components of complex nourishing medium on enzyme activity of strain 1760 S. commune.

Author Biographies

А.С. Бухало, M.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine

д.б.н., проф.

О.М. Дуган, National Technical University of Ukraine “Kiev Polytechnic Institute”

д.б.н., проф.

М.Р. Максимюк, National Aviation University

к.х.н., доц.

В.М. Ліновицька, National Technical University of Ukraine “Kiev Polytechnic Institute”

старш. викл.

References

Hummel K.M. Extracellular protease production by submerged cultures of Schizophyllum commune / Katrina M. Hummel, Amy L. Inselman, Erica R. Ramos et all // Mycologia. 1998. Vol. 90, N 5. - P. 883–889.

Pandee P. Production and properties of a fibrinolytic enzyme by Schizophyllum commune BL23 / Patcharaporn Pandee, Aran H-Kittikul, Ohsugi et all // Songklanakarin J. Sci. Technol. - 2008. – Vol. 30, N 4. – P. 447 453.

Subramaniyan S. Biotechnology of Microbial Xylanases: Enzymology, Molecular Biology, Application / S. Subramaniyan, P. Prema // Critical Reviews in Biotechnology. 2002. – Vol. 22, N 1. – Р. 33–46.

Kolenová K. Purification and characteriza-tion of two minor endo-β-1,4-xylanases of Schizophyllum commune / Katarína Kolenová, Mária Vršanská, Peter Biely // Enzyme and Microbial Technology. - 2005. - Vol. 36, N 7. – P. 903 910.

Wu J. -glucosidase derived from Schizophyllum commune an efficient enzyme of biotransformation of genistein / J. Wu, W. Yu, X. Zhang // Book of abstracts the 5th International Medicinal Mushroom Conference. Nantong, China, 5-8 September, 2009. – P. 139-140.

Baldrian P. Fungal laccases: occurrence and properties / P. Baldrian // FEMS Microbial Rev. - 2006. – Vol. 30. – P. 215-242.

Kenkebashvilli N. Effect of nutrient medium composition on laccase and manganese peroxidase activity in medicinal mushrooms / N. Kenkebashvilli, V. Elisashvilli, Y. Hadar // International Journal of Medicinal Mushrooms. - 2009. - Vol. 11, N 2. - P. 191-198.

Molitoris H.P. Physiology of marine fungi. A screening program for marine fungi / H.P. Molitoris, K. Schaumann // The biolgy of marine fungi. Eds. S.T. Moss. – Cambrige : Cambrige University Press, 1986. – P. 35-47.

Бухало А.С. Высшие съедобные базидио-мицеты в чистой культуре / А.С. Бухало. – К.: Наукова думка, 1988. – 144 с.

Дудка И.А. Методы экспериментальной микологии: справ. / И.А. Дудка, С.П. Вассер, И.А. Элланская и др. – К.: Наукова думка, 1982. – 561 с.

Тодоров И.И. Клинические лабораторные исследования в педиатрии / И.И. Тодоров. – София: Медицина и физкультура, 1968. – 230 с.

Гавриленко В.Ф. Большой практикум по физиологии растений / В.Ф. Гавриленко, М.Е. Ладыгина, Л.М. Хандобина. – М.: Высш. шк., 1975. – С. 284-285.

How to Cite

Бухало, А., Дуган, О., Максимюк, М., & Ліновицька, В. (2012). ENZIME ACTIVITY OF HIGHER BASIDIOMYCETES MUSHROOM SCHIZOPHYLLUM COMMUNE. Proceedings of National Aviation University, 52(3), 154–159. https://doi.org/10.18372/2306-1472.52.2369

Issue

Section

BIOTECHNOLOGY