METHOD OF LIQUID CHROMATOGRAPHY – TANDEM MASS-SPECTROMETRY FOR ENNIATINS A, A1, B, B1 AND BEAUVERICIN DETERMINATION IN WINTER WHEAT
Keywords:beauvericin, enniatins, Fusarium spp., liquid chromatography – tandem mas-spectrometry, mycotoxins
Objective: the aim of this study is to develop and validate a method of liquid chromatography – tandem mass-spectrometry for determination Fusarium mycotoxins namely eniatins A, A1, B, B1 and beauvericin. Methods: this article considers an analytical method based on high-performance liquid chromatography coupled with tandem mass-spectrometry for quick separation, identification, and quantification of mycotoxins in one sample. Results: it is proved that proposed method is accurate, selective, and rapid for identification and quantification of law-molecular weight compound accumulated in grain in small amount. Discussion: the effectiveness of liquid chromatography – tandem mass-spectrometry for determination of low concentrated mycotoxins in samples is confirmed. The validation ranges for analysts are compared to similar aimed researches of past years.
Ministry of Agrarian Policy and Food of Ukraine (2017), “A sown area under crop in 2017”, available at: www.minagro.gov.ua (Accessed 13 June 2017).
Brakhage A.A. (2013) Regulation of fungal secondary metabolism. National Review. Microbiology, No 11, pp. 21–32.
Peraica M., Radić B., Lucić A., Pavlović. M (1999) Toxic effects of mycotoxins in humans. Bulletin of the World Health Organization, vol. 77, No. 9, pp. 754-766.
Oueslati, S., Meca, G., Mliki, A., Ghorbel, A., Mañes, J. (2011). Determination of Fusarium mycotoxins enniatins, beauvericin and fusaproliferin in cereals and derived products from Tunisia. Food Control, No 22, pp. 1373–1377. doi: 10.1016/j.foodcont.2011.02.015.
Kirst H. A., Yeh W.-K., Zmijewski M.J., Jr (2001) Enzyme Technologies for Pharmaceutical and Biotechnological Applications New York, Marcel Dekker, Ink., 615 p.
Butt T. M., Jackson C., Magan N. (2001) Fungi As Biocontrol Agents: Progress Problems and Potential Wallingford, CABI Publishing, 393 p.
Serrano A.B. (2015) Development of a rapid LC-MS/MS method for the determination of emerging Fusarium mycotoxins enniatins and beauvericin in human biological fluids. Toxins, No 7, pp. 3554-3571.
Uhlig S., Ivanova L. (2004) Determination of beauvericin and four other enniatins in grain by liquid chromatography – mass spectrometry. Journal of chromatography A, No 1050, pp. 173-178. doi: 10.1016/j.chroma.2004.08.031.
PubMed Single Citation Matcher. Available at: https://www.ncbi.nlm.nih.gov/pubmed/citmatch
Bolechová M., Benesova K., Belakova S., Caslavsky J., Pospichalova M., Mikulikova R. (2015) Determination of seventeen mycotoxins in barley and wheat in the Czech Republic. Food Control, No 47, pp. 108-113. doi: 10.1016/j.foodcont.2014.06.045.
Berthiller F. (2007) Chromatographic methods for the simultaneous determination of mycotoxins and their conjugates in cereals. International Journal of Food Microbiology, No 119, pp. 33-37.
Jestoy M. (2008) Emerging fusarium-mycotoxins fusaproliferin, beauvericin, enniatins, and moniliformin: a review. Critical review in food science and nutrition, No 48, pp. 21-49. doi: 10.1080/10408390601062021.
Habler K, Rychlik M. (2016) Multi-mycotoxin stable isotope dilution LC-MS/MS method for Fusarium toxins in cereals. Analytical and Bioanalytical Chemistry, No 408, pp. 307-317. doi: 10.1007/s00216-015-9110-7.