INFLUENCE OF MICRODOSE OF FATTY ACIDS OF CITRULLUS COLOCYNTHIS EXTRACT ON LIPOPROTEIDS OF RAT BLOOD WITH DIABETES MELLITUS TYPE 1
DOI:
https://doi.org/10.18372/2306-1472.73.12181Keywords:
cholesterol, Сitrullus colocynthis, diabetes mellitus, fatty acids, lipoproteins, triglyceridesAbstract
Purpose: To investigate the effect of microdoses of fatty acids (FA) of partially defatted dry Citrullus colocynthis extract on the lipid metabolism of rats with type 1 diabetes mellitus (DM) has been studied. Methods: Raw materials were degreased by extraction in a Soxhlet apparatus under conditions of shortening the evaporation time. With the help of gas-liquid chromatographic research, trace concentrations of FA were revealed in the composition of the obtained dry extract of Citrullus colocynthis. Results: It was found that the introduction of the extract of the Citrullus colocynthis vegetal solution with a dose of 400 mg/kg to rats causes redistribution of the classes of lipoproteins to the values of the control group. The dose of 200 mg/kg – does not cause statistically significant changes in plasma lipoproteins. Conclusions: Finally the Citrullus colocynthis extract containing micro doses of FA in their uniform distribution has a positive effect on lipid metabolism, does not bring to an increase of level of the total cholesterol, does not cause an increase of body weight.
References
Tron'ko N.D., Sokolova L.K., Vlasenko M.V., Kostyukevich A.A. (2015) Dostizhenie celej lecheniya pacientami s saxarny'm diabetom v Ukraine. Rezul'taty' Mezhdunarodnogo issledovaniya po izucheniyu praktiki vedeniya saxarnogo diabeta (IDMPS) [Achieving the goals of treatment for patients with diabetes in Ukraine. The results of the International Study on the Practice of Diabetes Mellitus (IDMPS)]. Endokry`nologiya, no. 4, pp. 658–668. (In Russian)
Chekina N.A., Chukaev S.A., Nikolaev S.M. (2010) Saxarny'j diabet: vozmozhnosti farmakoterapii s ispol'zovaniem sredstv rastitel'nogo proisxozhdeniya [Diabetes mellitus: the possibilities of pharmacotherapy with the use of herbal products] Vestnik Buryatskogo universiteta, no. 12, pp. 71–78. (In Russian)
Verma J., Rathore D.S., Agarwal S., Tripathi V. (2015) Effects of Citrullus colocynthis and Cucumis callosus extract on blood glucose levels in alloxan-induced diabetic rats. SGVU Int. J. Env. Sci. Technol, vol. 1, no. 1, pp. 50–56.
Hussain A.I., Rathore H.A., Sattar M.Z. [et al.] (2014) Citrullus colocynthis (L.) Schrad (bitter apple fruit): a review of its phytochemistry, pharmacology, traditional uses and nutritional potential. J. Ethnopharmacol, vol. 155, no. 1, pp. 54–66.
Shi C., Karim S., Wang C. [et al.] (2014) A review on antidiabetic activity of Citrullus colocynthis Schrad. Acta Pol. Pharm, vol. 71, no. 3, pp. 363–367.
Marwat S.K., Rehman F., Khan E.A. [et al.] (2014) Useful ethnophytomedicinal recipes of angiosperms used against diabetes in South East Asian Countries (India, Pakistan & Sri Lanka). Pak. J. Pharm. Sci, vol. 27, no. 5, pp. 1333–1358.
Hii C.S., Howell S.L. (1985) Effects of flavonoids on insulin secretion and 45Ca2+ handling in rat islets of Langerhans. J. Endocrinol, vol. 107, no. 1, pp. 1–8.
Virot M., Tomao V., Colnagui G. [et al.] (2007) New microwave-integrated Soxhlet extraction. An advantageous tool for the extraction of lipids from food products. J. Chromatogr. A., vol. 1174, no. 1-2, pp. 138–144.
Lamazyan G.R. (2015) Gipoglikemichna akty`vnist` suxogo ekstraktu plodiv Citrullus Colocynthis (L.) Shrad [Hypoglycemic activity of dry extract of fruits Citrullus Colocynthis (L.) Shrad]. Farmacevty`chny`j zhurnal, no. 5, pp. 90–94. (In Ukrainian)
Bazarnova M.A., Gette Z.P. (1994) Klinichna laboratorna diagnosty`ka. Prakty`chni zanyattya z klinichnoyi bioximiyi [Clinical laboratory diagnostics. Practical classes in clinical biochemistry]. Ky`yiv, Vy`shha shkola Publ., 424 p. (In Ukrainian)