https://jrnl.nau.edu.ua/index.php/ecobiotech/issue/feedProblems of Environmental Biotechnology2023-12-12T15:24:21+02:00Drazhnikova Annadrazhnikova@nau.edu.uaOpen Journal Systems<p>Online scientific journal “Problems of Environmental Biotechnology” was founded by National Aviation University in 2012. The journal reviews a wide range of theoretical and applied problems of environmental biotechnology in biological and technical branches of science.</p> <p>Language: Ukrainian, English.</p>https://jrnl.nau.edu.ua/index.php/ecobiotech/article/view/18102Anti-fouling properties of a protective coating based on alkyd enamel modified with a hydrophobic cationic biocide2023-12-12T15:01:07+02:00S.P. Rogalskysergey.rogalsky@gmail.comO.V. Moshynetsmoshynets@nas.gov.uaO.V. Dzhuzhasergey.rogalsky@gmail.comO.P. Tarasyuksergey.rogalsky@gmail.comI.O. MorozovskaMorozovska@nas.gov.uaO.O. ProtasovProtasov@nas.gov.ua<p>New hydrophobic cationic biocide 1-dodecylpyridinium dodecylbenzenesulfonate (PyrC<sub>12</sub>-DBS) has been synthesized and evaluated as antifouling additive for protective coating based on alkyd enamel. Protective coatings, containing 10 weight percent of cationic biocide, were found to have high resistance to biofouling by aqueous micro- and macroorganisms.</p>2023-12-12T00:00:00+02:00Copyright (c) 2023 https://jrnl.nau.edu.ua/index.php/ecobiotech/article/view/18101Antibacterial activity of the cationic biocide 1-dodecylpyridinium tetrafluoroborate against multidrug-resistant strain of Acinetobacter baumannii2023-12-12T14:48:27+02:00S.P. Rogalskysergey.rogalsky@gmail.comD.M. Godynasergey.rogalsky@gmail.comI.V. Semeniutasergey.rogalsky@gmail.comO.P. Tarasyuksergey.rogalsky@gmail.comL.O. Metelytsiasergey.rogalsky@gmail.com<p>Hydrophobic cationic biocide 1-dodecylpyridinium tetrafluoroborate (PyrC<sub>12</sub>-BF<sub>4</sub>) has been synthesized. The antibacterial activity of cationic biocide against antibiotic-resistant strains-isolates of <em>Acinetobacter baumannii</em> has been established. According to results of molecular docking, the inhibition of the active site FabG may be one of the possible mechanisms of anti-<em>A. baumannii</em> activity of the PyrC<sub>12</sub>-BF<sub>4</sub>.</p>2023-12-12T00:00:00+02:00Copyright (c) 2023 https://jrnl.nau.edu.ua/index.php/ecobiotech/article/view/18100Features of growing energy crop Camelina using the biological product "Baikal EM"2023-12-12T14:39:58+02:00D.V. Liapyn5782018@stud.nau.edu.uaI.M. Korniienkoirina.kornienko.1979@gmail.comO.O. Kuznietsovaekyznec@ukr.netO.Y. Filimonenkoddtu.kafpbt@ukr.netA.L. Kovalenkoirina.kornienko.1979@gmail.com<p>A study of the peculiarities of growing the energy crop Camelina using the biological product "Baikal EM".</p>2023-12-12T00:00:00+02:00Copyright (c) 2023 https://jrnl.nau.edu.ua/index.php/ecobiotech/article/view/18098The significance of the introduction of plants for optimizing the state of tree ecosystems in the Dniprovian steppe of Ukraine2023-12-12T14:03:51+02:00M.O. Kvitkok_zoology@kdpu.edu.uaO.A. Likholatumsf.tourism.grs@gmail.comT.Y. Lykholatlykholat2006@ukr.netY.S. Borodailykholat2006@ukr.netY.V. Lykholatlykholat2006@ukr.net<p>Regional environmental safety issues have recently played a special role. Crimes against nature in the future should be considered with special care, since they have negative consequences for human existence in any region. Therefore, the scientific basis of regional environmental safety is necessary for the development of any social projects.</p>2023-12-12T00:00:00+02:00Copyright (c) 2023 https://jrnl.nau.edu.ua/index.php/ecobiotech/article/view/18097Investigation of the dynamics of action optima for some physicochemical factors in the course of complex optimization of conditions for the biosynthesis of amylolytic enzymes of microbial origin2023-12-12T13:55:13+02:00Y.M. Ivchenkoivchenko_ym@czvfn.dnu.edu.uaT.P. Kilochokivchenko_ym@czvfn.dnu.edu.uaN.B. Mitinaivchenko_ym@czvfn.dnu.edu.ua<p>The most important factors in increasing efficiency are the composition of the nutrient medium, which can be optimized depending on the needs of microorganisms, and physicochemical factors that directly affect both the biosynthesis of amylolytic enzymes by the studied strain and the biosynthetic capacity of the strain in general. The aim of this work is to optimize the biosynthetic capacity of <em>Streptomyces recifensis</em> var. <em>lyticus</em> 2P-15 strain in terms of amylolytic enzyme synthesis. The object of study is the strain of <em>Streptomyces recifensis</em> var. <em>lyticus</em> 2P-15, obtained by three-stage selection of the producer. The simplex method of selecting the composition of the medium was used to perform the study.</p>2023-12-12T00:00:00+02:00Copyright (c) 2023 https://jrnl.nau.edu.ua/index.php/ecobiotech/article/view/18096New biological tools for the study of plant systems in modern biotechnology2023-12-12T13:46:32+02:00L.I. BronnikovaZlenko_lora@ukr.netI.O. ZaitsevaZlenko_lora@ukr.net<p>Genetic control is exerciesed at all stages of growth and development of the plant system. The plant metabolism is coordinated according to the integraction of direct and cross-talk between al vital systems, which are established and constantly evolve in the from of dynamic phenotypic effects. The acquisition of biological information improvement of methods and research.</p>2023-12-12T00:00:00+02:00Copyright (c) 2023 https://jrnl.nau.edu.ua/index.php/ecobiotech/article/view/18090European practices of bioenergy development for the modern world2023-12-11T17:19:32+02:00V.I. Karpenkovalerii.karpenko@npp.nau.edu.uaI.M. Korniienkoirina.kornienko.1979@gmail.comE. Caceres Cabanavalerii.karpenko@npp.nau.edu.ua<p>European practices of bioenergy development for the modern world are considered.</p>2023-12-12T00:00:00+02:00Copyright (c) 2023 https://jrnl.nau.edu.ua/index.php/ecobiotech/article/view/18089Study of protective properties of mucous substances of psyllium and chia seeds on probiotic cultures in bread-making technology2023-12-11T16:42:36+02:00M.V. Kryzhova6915798@stud.nau.edu.uaI.M. Korniienkoirina.kornienko.1979@gmail.comV.M. Huliaievvgulyaev@dstu.dp.uaY.M. Korniienkoirina.kornienko.1979@gmail.com<p>The technology of preparation of bread sourdough starter with improved functional characteristics due to the addition of psyllium and chia seeds was investigated.</p>2023-12-12T00:00:00+02:00Copyright (c) 2023 https://jrnl.nau.edu.ua/index.php/ecobiotech/article/view/18088Technology of production of capsule forms of medicines in Ukraine2023-12-11T16:31:59+02:00A.M. Vizer5662758@stud.nau.edu.uaI.V. Honcharenkoigorgoncharenko2711@gmail.com<p>The technology of production of capsule forms of medicines in Ukraine is considered.</p>2023-12-12T00:00:00+02:00Copyright (c) 2023 https://jrnl.nau.edu.ua/index.php/ecobiotech/article/view/18087Energy-informational properties of hydrolates and their effect on Bacillus bacteria2023-12-11T12:56:06+02:00K.G. Garkavaimmunolog@ukr.netV.V. Kuskova5708999@stud.nau.edu.uaN.I. Dzhurenkoimmunolog@ukr.netO.M. Gorelovimmunolog@ukr.net<p>In the present work, hydrolates were obtained from different plants: medicinal lemon balm (<em>Melissa officinalis</em> L.), medicinal hyssop (<em>Hyssopus officinalis</em> L. ), <em>Tagetes erecta</em> L., <em>Sambucus nigra</em> L., and <em>Ginkgo biloba</em> L., and studied their effect on the activity of bacteria of the genus <em>Bacillus</em>. The energy information properties of hydrolates were studied by the biolocation method.</p>2023-12-12T00:00:00+02:00Copyright (c) 2023 https://jrnl.nau.edu.ua/index.php/ecobiotech/article/view/18086Circular economy principles in the bioconversion of vegetable waste into biofertilizer2023-12-11T12:46:23+02:00V.V. Kermash6892136@stud.nau.edu.uaI.M. Korniienkoirina.kornienko.1979@gmail.comO.O. Kuznietsovaekyznec@ukr.netL.S. Yastremskalarysa.yastremska@npp.nau.edu.uaM.M. Baranovskyyizbarvinok@gmail.comT.V. Kuz 5tanjasha66@gmail.com<p>A study was conducted on the processing of food waste, mainly vegetable waste, using the modern biopreparation "Baikal-EM" in 5 days. The effectiveness of this preparation has been proven, provided that expired bread is added in an amount of at least 10% of the total waste. The results of the research showed an increase in the biomass of fermenting microflora by 18%, which indicates that bread is a complete substrate for the growth and maintenance of working biomass, as it meets the nutritional needs.</p>2023-12-12T00:00:00+02:00Copyright (c) 2023 https://jrnl.nau.edu.ua/index.php/ecobiotech/article/view/18085European experience of waste management in the context of transition to a circular economy2023-12-11T12:31:52+02:00O.O. Kuznietsovaekyznec@ukr.netM.M. Baranovskyyizbarvinok@gmail.comI.M. Korniienkoirina.kornienko.1979@gmail.comL.S. Yastremskalarysa.yastremska@npp.nau.edu.ua<p>The European experience of waste management in the context of the transition to a circular economy is considered.</p>2023-12-12T00:00:00+02:00Copyright (c) 2023 https://jrnl.nau.edu.ua/index.php/ecobiotech/article/view/18084Using biomonitoring to assess the ecological state of soil in a technologically loaded area2023-12-11T12:22:45+02:00L.M. Cherniaklarysa.cherniak@npp.nau.edu.uaO.I. Proskurnia5647881@stud.nau.edu.uaO.M. Mikhieievoleksandr.mikhieiev@npp.nau.edu.uaT.I. Dmytrukhatetiana.dmytrukha@npp.nau.edu.ua<p>The aim of the study was to investigate the dependence of the growth characteristics of different plant species on the type of oil product that enters the soil.</p>2023-12-12T00:00:00+02:00Copyright (c) 2023 https://jrnl.nau.edu.ua/index.php/ecobiotech/article/view/18064Modifiers of microbial hydrolases among new types of biscytrate germanates2023-12-06T19:18:35+02:00O.V. Gudzenkoov_gudzenko@bigmir.netN.V. Borzovanv_borzova@bigmir.netL.D. Varbanetsov_gudzenko@bigmir.net<p>The modifiers of microbial hydrolases among new types of biscytrate germanates are considered.</p>2023-12-12T00:00:00+02:00Copyright (c) 2023 https://jrnl.nau.edu.ua/index.php/ecobiotech/article/view/18063Purification and physicochemical properties of Bacillus sp. IMB B 7883 proteases2023-12-06T19:10:35+02:00L.D. Varbanetsov_gudzenko@bigmir.netO.V. Gudzenkoov_gudzenko@bigmir.net<p>The aim of the work was to isolate and purify these enzymes from the supernatant of the culture liquid of the producer strain <em>Bacillus</em> sp. IMV B-7883, as well as to study their physical and chemical properties and substrate specificity. Isolation and purification of the proteolytic complex of <em>Bacillus</em> sp. IMV B-7883 was carried out by classical biochemical methods: precipitation of the supernatant of the culture liquid with ammonium sulfate of 90% saturation, gel permeation and ion exchange chromatography and rechromatography on Sepharose 6B. As a result, elastase activity was increased by 63.6 times (4138 U/mg of protein) and fibrinogenolytic activity by 44.07 times (833 U/mg of protein). Like enzymes from other well-known producers, the investigated proteases were of low molecular weight, 23 and 20 kDa, respectively, for the protease with elastolytic and fibrinogenolytic activity. Unlike the elastases described in the literature, which are alkaline proteases, the enzyme with elastase activity had a pH-optimum of 7.0, while the enzyme with fibrinogenolytic activity was an alkaline protease with a pH-optimum of 8.0. Research on substrate specificity of enzymes showed the protease with elastase activity hydrolyzed only elastin, while the protease with fibrinogenolytic activity, in addition to fibrinogen, showed specificity also for fibrin and, in trace amounts, for collagen.</p>2023-12-12T00:00:00+02:00Copyright (c) 2023 https://jrnl.nau.edu.ua/index.php/ecobiotech/article/view/18062Agar as a raw material for pharmaceuticals in capsule forms2023-12-06T18:54:05+02:00I.V. Honcharenkoigorgoncharenko2711@gmail.comA.M. Vizer5662758@stud.nau.edu.ua<p>Agar is considered as a raw material for drugs in capsule forms.</p>2023-12-12T00:00:00+02:00Copyright (c) 2023 https://jrnl.nau.edu.ua/index.php/ecobiotech/article/view/18061Energy aspects of oriental phytotherapy2023-12-06T18:48:00+02:00O.M. GorelovGorelov.O.M@nas.gov.ua<p>The energy aspects of oriental phytotherapy are considered.</p>2023-12-12T00:00:00+02:00Copyright (c) 2023 https://jrnl.nau.edu.ua/index.php/ecobiotech/article/view/18060Determination of the influence of viral infection on the sowing qualities of vegetable seeds2023-12-06T18:36:22+02:00V.O. Tsvihunagroecologynaan@gmail.comI.I. Humeniukgumenyuk.ir@gmail.comA.S. Levishkоagroecologynaan@gmail.com<p>The aim of the study was to determine the presence of viruses in the seeds of Solanaceae plants and their impact on seed quality.</p>2023-12-12T00:00:00+02:00Copyright (c) 2023 https://jrnl.nau.edu.ua/index.php/ecobiotech/article/view/18059Use of microbial exopolysaccharides in various industries2023-12-06T18:29:48+02:00O.A. Vintoniakoleksandra.semenova97@gmail.comL.R. Reshetnyakludres@ukr.net<p>Study the possibility of using specific and non-specific functions and properties of exopolysaccharides in practice.</p>2023-12-12T00:00:00+02:00Copyright (c) 2023 https://jrnl.nau.edu.ua/index.php/ecobiotech/article/view/18058Production features of the drug "Acylact"2023-12-06T17:04:45+02:00O.Y. Rusnak5298803@stud.nau.edu.ua<p>The peculiarities of production of the drug "Acylact" are considered.</p>2023-12-12T00:00:00+02:00Copyright (c) 2023